Bub1 (BUB1 Budding uninhibited by Benzimidazoles 1 Homolog, BUB1 Budding uninhibited by Benzimidazoles 1Homolog (yeast), BUB1A, BUB1L, Budding uninhibited by Benzimidazoles 1 (yeast Homolog), Budding uninhibited by Benzimidazoles 1 Homolog, hBUB1, Mitotic Checkpointserine/Threonine Protein Kinase BUB1, Mitotic Spindle Checkpoint Kinase, Putativeserine/Threonine Protein Kinase)

Size

£563.00

SKU:: 253951
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Fusion protein, corresponding to aa281-419 of Human Bub 1. Species sequence homology: Rat, Xenopus laevis, Kangaroo
Clone:: 14H5
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice