S-tag (Biotin)

Size

£691.00

SKU:: 228249
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant S-tag protein
Clone:: SBSTAGa
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the interest proteins, which are expressed in certain vectors containing the particular tagged fragments. The small tags are used to be cloned 50 along with the target proteins, and the antibodies against those fusion-tags are used to monitor fusion protein expression and purification. S-Tag is an epitope tag composed of a 15aa peptide (KETAAAKFERQHMDS) derived from the pancreatic ribonuclease A. This antibody can be used to check S- tagged expressed proteins. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.