MMP-9 (Matrix Metalloproteinase 9) (HRP)

Size

£509.00

SKU:: 228246
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to MMP-9.
Clone:: SB15c
Format:: Supplied as a liquid in 1ml PBS, pH 7.4, 50% glycerol. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:: Matrix metalloproteinases (MMPs) are a family of at least 20 structurally related, zinc-containing enzymes that have the ability to breakdown connective tissue.MMP-9 is also known as gelatinase B and 92kD gelatinase. MMP-9, along with MMP-2, belongs to the gelatinase group of MMP’s which have three repeats of a type II fibronectin domain inserted in the catalytic domain. MMP-9 has shown direct and indirect substrate specificity to collagens IV, V, X and XIV, gelatin, elastin, proteoglycans, plasminogen, aggrecan, galectin-3, entactin, fibrillin, fibrin, IL-1b, amyloid b peptide and myelin basic protein. 1-15 Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:4000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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