Fas Ligand (FITC)

Size

£389.00

SKU:: 228200
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Fas Ligand.
Clone:: SB93a
Format:: Supplied as a liquid in PBS, sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: Fas Ligand (CD178) is a 40kD type II transmembrane protein that is a member of the tumor necrosis factor superfamily. This well characterized potent apoptotic factor is utilized by cytotoxic T cells and natural killer cells to selectively kill virus infected and tumorigenic cells.1,2 Moreover, studies indicate that Fas Ligand is an important regulator in immune homeostasis where it has been shown to down-regulate immune responses during activation-induced cell death.3 Fas Ligand initiates apoptosis by binding to its receptor (CD95) on target cells which facilitates recruitment of numerous signaling proteins to form a death inducing signaling complex. This signaling complex is then believed to propagate the apoptotic signal through the recruitment and activation of Caspase-8.4 The monoclonal antibody detects a band at ~40kD, corresponding to the transmembrane (insoluble) form of Fas Ligand. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice