228199
Fas Ligand (CD178)
Size
£364.00
- SKU:
- 228199
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Fas Ligand.
- Clone:
- SB93a
- Format:
- Supplied as a liquid in BBS, pH 8.2. No preservative or amine-containing buffer salts added.
- Extra Details:
- Fas ligand (CD178) is a 40 kD type II transmembrane protein that is a member of the tumor necrosis factor superfamily. This well characterized potent apoptotic factor is utilized by cytotoxic T cells and natural killer cells to selectively kill virus infected and tumorigenic cells. Moreover, studies indicate that Fas ligand is an important regulator in immune homeostasis where it has been shown to down-regulate immune responses during activation-induced cell death. Fas ligand initiates apoptosis by binding to its receptor, CD95, on target cells which facilitates recruitment of numerous signaling proteins to form a death inducing signaling complex. This signaling complex is then believed to propagate the apoptotic signal through the recruitment and activation of caspase-8. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1ug/10e6 cells Western Blot: 0.5-2ug/ml, detects a band at ~40kD corresponding to the transmembrane/insoluble form of Fas ligand. Immunoprecipitation: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice