Erk1/2 (Erk1, Erk2, MAPK)

Size

£422.00

SKU:: 228195
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Erk1 and Erk 2.
Clone:: SB46b
Format:: Supplied as a liquid in 1ml BBS, pH 8.2. No preservatives or amine-containing buffer salts added.
Extra Details:: Erk1 and Erk2 are factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases 2-5, Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85% identical and have higher identity in the core regions involved in substrate binding 5. Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2 5,6. Both sites must be phosphorylated for maximum activity 2-5. These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus 2-4. Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 5ug/ml Immunoprecipitation: 10-15ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice