Cytokeratin 8 (Keratin 8)

Size

£448.00

SKU:: 228191
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Keratin 8 at C-terminal.
Clone:: SB37b
Format:: Supplied as a liquid in 1ml BBS, pH 8.2. No preservative or amine-containing buffer salts added.
Extra Details:: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology.1-5 Keratins demonstrate tissue- and differentiation- specific expression profiles. Cytokeratin 8 (55kD) belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. It is expressed in all simple type epithelia tissues (e.g., liver, pancreas, kidney, gut epithelial lining) but not in stratified squamous epithelia. Cytokeratin 8 is also present in the majority of adenocarcinomas and ductal carcinomas but is absent in squamous cell carcinomas. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 2ug/ml Western Blot: 2ug/ml Immunohistochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice