Cytokeratin 18 (Keratin 18) (HRP)

Size

£512.00

SKU:: 228187
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Keratin 18 at N-terminal.
Clone:: SB38b
Format:: Supplied as a liquid in 50% PBS, pH 7.4, 50% glycerol. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology.1-5 Keratins demonstrate tissue- and differentiation- specific expression profiles. Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein of approximately 48kD. It exists as a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 is expressed in all simple type epithelia and basal cells of many squamous and non-epidermal epithelia, and is often co-expressed with cytokeratin 8.6-8 Tissues from the gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:2000 Western Blot: 1:2000-1:8000 Immunohistochemistry: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice