228174
CD8a (Ly-2, Lyt-2, Ly-35, Ly-B, T8) (APC/Cy7)
Size
£480.00
- SKU:
- 228174
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Spleen cells or thymocyte membranes.
- Clone:
- 53-6.7
- Format:
- Supplied as a liquid in PBS, sodium azide. Labeled with Allophycocyanin (APC/Cy7).
- Extra Details:
- In the mouse, CD8 exists in two forms–a CD8 heterodimer composed of an a chain (CD8a) and a b chain (CD8b); and (ii) a homodimer of two a chains.The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I- restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the monoclonal antibody effectively depletes CD8a+ cells. I MHC antigens and T cell responses to IL-2. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC/Cy7 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice