228171
CD86 (B7-2, B70, Ly-58) (PE/Cy7)
Size
£486.00
- SKU:
- 228171
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- LPS-activated CBA/Ca mouse spleen B cells.
- Clone:
- GL1
- Format:
- Supplied as a liquid in PBS, sodium azide. Labeled with R-Phycoerythrin (PE/Cy7).
- Extra Details:
- CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. CD86, along with CD80 (B7-1), is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152 (CTLA- 4). Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. The monoclonal antibody blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE/Cy7 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice