CD86 (B7-2, B70, Ly-58) (APC)

Size

£486.00

SKU:: 228169
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: Mouse B cell lymphoma cell line 5C2.
Clone:: 2D10
Format:: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Extra Details:: CD86 (B7-2) is an 80kD type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80 (B7-1), CD86 participates in T cell costimulation via interaction with CD28 and CD152 (CTLA-4). It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes, and dendritic cells. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.