228167
CD8 (PE/Cy5)
Size
£466.00
- SKU:
- 228167
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Chicken thymocytes and Ig-negative blood leukocytes.
- Clone:
- CT-8
- Format:
- Supplied as a liquid in PBS, sodium azide. Labeled with R-Phycoerythrin (PE/Cy5).
- Extra Details:
- In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two a chains and (ii) a heterodimer of an a chain and a b chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8a- and CD8b-chains, a relatively large proportion of the CD8+ TCRgd cells in the spleens of embryos and young chicks express only the a-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgd CD8ab cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. Recognizes the CD8a chain and has been shown to react to a polymorphic determinant in turkey. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.2ug/10e6 cell Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE/Cy5 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice