228163
CD8 (Cy5)
Size
£484.00
- SKU:
- 228163
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Chicken thymocytes.
- Clone:
- 3-298
- Format:
- Supplied as a liquid in PBS, sodium azide. Labeled with Cy5.
- Extra Details:
- In the chicken the CD8 molecule is present in two forms - (i) a homodimer of two a chains and (ii) a heterodimer of an a chain and a b chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow.While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8a- and CD8b-chains, a relatively large proportion of the CD8+ TCRgd cells in the spleens of embryos and young chicks express only the a-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgd CD8ab cells in the chicken that is not found in rodents. The monoclonal antibody recognizes the CD8a chain of both the chicken and turkey. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice