CD8 (Cy5)

Size

£484.00

SKU:: 228162
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Chicken thymocytes and Ig-negative blood leukocytes.
Clone:: CT-8
Format:: Supplied as a liquid in PBS, sodium azide. Labeled with Cy5.
Extra Details:: In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two a chains, and (ii) a heterodimer of an a chain and a b chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8a- and CD8b-chains, a relatively large proportion of the CD8+ TCR-y8 cells in the spleens of embryos and young chicks express only the a chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-y8 CD8ab cells in the chicken that is not found in rodents.1,3 Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow.1,Recognizes the CD8a chain and has been shown to react to a polymorphic determinant in turkey. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice