CD49b (VLA-2, Very Late Antigen-2, a2 Integrin) (APC)

Size

£460.00

SKU:: 228135
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rat
Immunogen:: NK cells isolated from C57BL/6 mice.
Clone:: DX5
Format:: Supplied as a liquid in PBS, sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: Reacts with CD49b, also known as very late antigen-2 (VLA-2) and a2 integrin. majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR).Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets ofNK-1.1- and NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes. NK cells cultured in the presence of IL-2 progressively lose reactivity as a consequence of cellular proliferation. Therefore, it can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody has not been demonstrated to have activating or blocking activity. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice