CD45 (LCA, T200, Ly5) (APC)

Size

£442.00

SKU:: 228116
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified T cells from human lymph nodes.
Clone:: F10-89-4
Format:: Supplied as a liquid in 1ml PBS, sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220kD) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul/106e cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.