CD3, epsilon (Cy5)

Size

£350.00

SKU:: 228084
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: IL-4 producing Th2 cell lines including D10
Clone:: C363.29B
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Cy5.
Extra Details:: CD3 a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody recognizes an epitope on the 25kD chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble can activate primed and naïve T cell in vitro. Immobilized monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The monoclonal antibody is cytolytic, easily used for cell surface staining, and a good immunoprecipitating. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Cy conjugates are sensitive to light.

Shipping Conditions:: Blue Ice