CD1.1

Size

£422.00

SKU:: 228036
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Lymphocytes from the bursa of Fabricius of outbred chickens.
Clone:: CB3
Format:: Supplied as a liquid in 1ml BBS, pH 8.2. No preservative or amine-containing buffer salts added.
Extra Details:: CD1 molecules, like MHC I and II, play an important role in the immune system by presenting lipid, glycolipid and lipopeptide antigen to T and NKT cells. The chicken CD1 gene is located in the chicken MHC B locus and has an important implication for the primordial MHC gene evolution. Two proteins have been identified - CD1.1 and CD1.2. Analysis of RNA from blood cells showed that both genes are expressed in Bu-1+ cells and CD8a+ cells. CD1.2 is expressed in TCRgd cells but is nearly undetectable in TCRab. The monoclonal antibody can identify cells of the B cell lineage and recognizes CD1.1 but not CD1.2 on the surface of transfected mammalian cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.