228035
Caspase-8
Size
£461.00
- SKU:
- 228035
- Application:
- WB
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Purified native human caspase-8.
- Clone:
- SB125a
- Format:
- Supplied as a liquid in BBS, pH 8.2. No preservatives or amine-containing buffer salts added.
- Extra Details:
- The caspases are a family of cysteine proteases that induce apoptosis in response to a variety of intra- and extracellular stimuli. These enzymes are synthesized as inactive precursors which become cleaved at aspartate-specific sites upon induction of apoptosis. Caspase-3 (CPP32, Yama, apopain) is one of the best characterized caspases in terms of specificity and function in apoptosis. It is a ubiquitously expressed protein in mammals and is highly prevalent in cells with lymphocytic origins, indicating a regulatory role in the immune system. Caspase-3 plays a critical role during embryonic development by inducing morphogenetic cell death in the mammalian brain and has also been linked to chromatin condensation, DNA fragmentation and blebbing. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: detects a band at ~55kD corresponding to the full length form of caspase-8. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice