228033
Caspase-3
Size
£341.00
- SKU:
- 228033
- Application:
- WB
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Caspase-3.
- Clone:
- SB71b
- Format:
- Supplied as a liquid in BBS, pH 8.2. No preservatives or amine-containing buffer salts added.
- Extra Details:
- The caspases are a family of cysteine proteases that induce apoptosis in response to a variety of intra- and extracellular stimuli. These enzymes are synthesized as inactive precursors which become cleaved at aspartate-specific sites upon induction of apoptosis. Caspase-3 (CPP32, Yama, apopain) is one of the best characterized caspases in terms of specificity and function in apoptosis. It is a ubiquitously expressed protein in mammals and is highly prevalent in cells with lymphocytic origins, indicating a regulatory role in the immune system. Caspase-3 plays a critical role during embryonic development by inducing morphogenetic cell death in the mammalian brain and has also been linked to chromatin condensation, DNA fragmentation and blebbing. The monoclonal antibody detects endogenous levels of full length caspase-3 (35kD) and the large fragment of caspase-3 resulting from cleavage (17kD). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice