Lysosomal Acid Lipase (Cholesteryl Ester Hydrolase, Cholesteryl Esterase, Lipase A, Sterol Esterase, LIPA) (FITC)

Size

£576.00

SKU:: 227796
Application:: FLISA
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Lysosomal acid lipase.
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: The Lipase gene family is part of one of the largest genetic superfamilies in living organisms. Members of the AB hydrolase subfamily all contain an enzyme core with an alpha/beta sheet, not barrel, of eight beta-sheets connected by alpha-helices. The AB hydrolase subfamily plays a crucial role in the metabolism of lipids. Members of this family include hepatic lipase (HL), endothelial lipase(EL), lipoprotein lipase (LPL), pancreatic lipase (PL), gastric lipase (GL), LCAT, and lysosomal acid lipase (LAL). LAL is the important enzyme in the hydrolysis of triglycerides and cholesteryl esters in lysosomes. LAL has six potential N-glycosylation sites and one potential O-glycosylation site, and it is mediated by macrophage mannose receptors. Defects in the LAL gene have been linked to Wolman disease (WD) and CE storage disease (CESD), while overexpression of LAL leads to atherosclerosis. Applications: Suitable for use in FLISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice