227447-ML650
MHC Class I (MaxLight 650)
Size
£837.00
- SKU:
- 227447-ML650
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Sheep T cells.
- Clone:
- VPM19
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Recognizes the ovine homologue of the human MHC Class I, a monomorphic determinant expressed on the heavy chain of sheep MHC Class I, (OLA Class I). The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In sheep, this is often referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC Class I functions in the recognition and presentation of foreign antigens to T-cells. Ovine MHC Class I is a membrane glycoprotein with a molecular weight of ~44kD, expressed on the cell surface of all peripheral blood leucocytes. Has been in used in studies on a number of domestic animal diseases, in particular Maedi Visna virus infection, a disease of significant inportance in commercial sheep flocks (Lee et al. 1996, Ryan et. al. 2000 and Wu et. al. 2008). Has been cited as recognising MHC class I in some other species and has been used in a study of Feline herpes virus infection (Montagnaro et. al. 2009). Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice