227444-ML490
Granulocytes (Neutrophil Lineage) (MaxLight 490)
Size
£832.00
- SKU:
- 227444-ML490
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Porcine bone marrow haematopoietic cells (BMHC).
- Clone:
- 6D10
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Recognizes a 60kD antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells. Expression of the antigen recognized decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, conjunction with other clones, allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: early myeloid precursors, immature neutrophils, mature neutrophils and mature eosinophils and basophils. Has been shown as suitable for use on cytospins (Perez et al. 2007). Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice