227427-APC

CD4a (APC)

Size

£921.00

SKU:: 227427-APC
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Leukocytes isolated from porcine gut lamina propria.
Clone:: MIL17
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Recognizes a 55kD porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4a cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haverson et al. 2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily. Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmuller et al. 1987) Stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inman et al. 2010). Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: The epitope recognized is reported to be sensitive to formaldehyde fixation and tissue processing. Acetone fixation is recommeneded for frozen sections. Paraffin sections are not recommeneded. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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