227417
CD4
Size
£576.00
- SKU:
- 227417
- Purity:
- Purified by Protein A affinity chromatography form tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Equine Thymocytes
- Clone:
- CVS4
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- CD4 is a 58kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells. A study undertaken using 227417, to identify CD4 on several wild african equid species, indicates that CD4 recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200. 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice