CD32-Like (FcRII)

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£576.00

SKU:: 227414
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Ovine macrophages.
Clone:: VPM63
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Recognizes a cell surface antigen of 40-42kD which is similar to the human cell surface antigen designated as CD32. It has therefore been hypothesized that may recognise a minor isoform of the ovine homologue of human CD32 (Hopkins, J. et. al. 1996). CD32 is one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. CD32, also known as the Fc gamma RII, acts as a low affinity receptor for IgG. The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. Has been shown to recognize an antigen expressed by macrophages and monocytes but not by lymphocytes and shows strong staining of alveolar macrophages in flow cytometry. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:25-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice