227410
CD25 (IL-2R alpha Chain) (PE)
Size
£832.00
- SKU:
- 227410
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Enriched and stimulated Canine T cells.
- Clone:
- P4A10
- Format:
- Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml ddH2O. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Recognises the canine homologue of the human CD25 cell surface antigen, also known at IL-2Ra, a glycoprotein of ~55kD expressed primarily by activated T lymphocytes (Abrams et al. 2010). The IL-2 receptor is composed of 3 subunits, an a chain (CD25), a b chain (CD122) and a y chain (CD132), CD25 functions as a low affinity receptor for IL-2. Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (Tregs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity. Previous studies of canine Tregs have utilized the mouse anti human CD25 monoclonal antibody, which has demonstrated cross reactivity to canine CD25 (Rissetto et al. 2010). However this clone has poor affinity for canine CD25. The development now offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody (Abrams et al. 2010). The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers (Paoloni et al. 2008), and in genetically related diseases of the hemopoitic system (Bauer et al. 2009). The generation provides an improved tool for the identification of canine CD25 and will facilitate studies in this species. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat; use 10ul to label 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice