CD11/CD18 (LFA-1)

Size

£1,068.00

SKU:: 227396
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 24
Format:: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Extra Details:: Recognizes an epitope of 174kD present on leukocyte integrins, CD11/CD18 or beta 2-type integrins. The leukocyte integrins are a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions. T cells use integrins in essentially all of their functions. Integrins become active following signalling through other membrane receptors, which cause both affinity alteration and an increase in integrin clustering. The monoclonal antibody 24 recognizes a structural feature and is strictly dependent upon the presence of Mg2+. The epitope is located within, or in close proximity to, the three conserved cation binding domains and therefore a measure of Mg2+ bound to the leukocyte integrins and thus reflects functionally active molecules. The epitope can be induced on polymorphonuclear leukocytes and on monocytes. Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody binding. The monoclonal antibody inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2- activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell proliferation. Furthermore the monoclonal antibody prevents “deadhesion” of receptor/ligand pairs, possible locking leukocyte integrins in an “active” conformation. Applications: Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice