C5b-9 (Membrane Attack Complex, MAC)

Size

£991.00

SKU:: 227395
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat C5b-9.
Clone:: 2A1
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: Recognizes rat C5b-9. The antibody was shown to compete with antibodies to human C9 for its binding site on the C5b-9 complex, indicating that the reactive epitope is located on the C9 molecule. C5b-9 membrane attack complexes are assembled from five precursor molecules in the serum. Proteolytic cleavage of C5 by C5 convertase generates C5b which initiates assembly of the C5b-9 complex. The last step of C5b-9 complex formation involves polymerization of C9 which accompanies insertion of the complex into the cell membrane. During formation of C5b-8 and C9 polymerization, neoantigens are generated which are unique to the C5b-9 complex and are not present on any of the individual native complex components. The complement regulatory proteins CD59 and complement S-protein can both prevent C5b-9 insertion into the cell membrane. The formed SC5b-9 complex is unable to attach to cells and is cytolytically inactive. C5b-9 is involved in the progression of chronic proteinuric renal disease by mediating continuous tubulointerstitial damage. Early tubulointerstitial injury in the remnant kidney can be improved when C5b-9 complex forming is abrogated. The monoclonal antibody was raised against a rat C5b-9 neoantigen. Monoclonal antibody can be used as a coating antibody to detect C5b-9 in plasma and urine samples. Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA Capture: 30ug/ml in 50mM cabonate buffer pH 10.6 for 16 hours at 4°C Western Blot: Non-reduced Band at >200kD Immunohistochemistry (Formalin): Pretreat with Protease type XXIV for 10 minutes at 37°C before incubation Immunohistochemistry (Frozen): Fixed in acetone for 10 minutes at RT before incubation. Optimal dilutions to be determined by the researcher. Positive Control: Glomeruli of rats treated with anti-Thy-1.1 antibodies Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice