NKX3-1 (Homeobox Protein Nkx-3.1, Homeobox Protein NK-3 Homolog A, NKX3.1, NKX3A)

Size

£704.00

SKU:: 227378
Purity:: Purified by Protein A affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Purified recombinant protein corresponding to the amino-terminal 153 amino acids of human NKX3.1.
Format:: Supplied as a lyophilized powder. Reconstitute the antibody by resuspending in 1ml of sterile deionized water.
Extra Details:: NKX3.1 is located on human chromosome 8p21.21. It is an androgen regulated homeodomain gene with the expression of the NKX3.1 protein predominantly localized to prostate epithelial cells. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml in Tris or PBS, pH 7.3 with 2% carrier protein. Immunohistochemistry: 1ug/ml. For formalin-fixed paraffin embedded tissues, perform high- temperature antigen retrieval in 1mM EDTA, pH 8.0. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.