226958
Heat Labile Enterotoxin
Size
£626.00
- SKU:
- 226958
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Heat-labile enterotoxin.
- Clone:
- 15B343
- Format:
- Supplied as a liquid in PBS, pH 7.4, 3mM KCl,1.5mM KH2PO4,140mM sodium chloride, 8.0mM Na2HPO4.
- Extra Details:
- Heat-labile enterotoxin is a type of labile toxin found in Escherichia coli that is inactivated at high temperatures. It acts similarly to the cholera toxin by raising cAMP levels through ADP-ribosylation of the alpha-subunit of a Gs protein leading to the constitutive activation of adenylate cyclase. Elevated cAMP levels stimulate secretion of Chloride ions and water from the enterocyte into the gut lumen, thus causing watery diarrhea. In addition to its effects on chloride secretion, which involve the same steps as the effects of cholera toxin, heat-labile enterotoxin binds additional substrates: lipopolysaccharide on the surface of E. coli cells and A-type blood antigens. The importance of these binding events is not yet known. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice