Ac-Arg-Leu-Arg-AMC (Ac-RLR-AMC, Proteasome Substrate)

Size

£266.00

SKU:: 226820
Molecular Weight:: 642.8
Purity:: ≥97% (HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
CAS Number:: 929903-87-7
Format:: Supplied as a solid lyophilized powder.
Extra Details:: Fluorogenic tri-peptide substrate for measuring the trypsin-like peptidase activity of the 20S proteasome. It is cleaved exclusively at the amide R-AMC bond to release fluorescent product. Shows low Km and high specific activity. This substrate is useful for inhibitor screening and kinetic analysis. CAS No.: 929903-87-7 (free base) Excitation: Excitation: 380nm. Emission: 460nm. Molecular Formula: C30H46N10O6 Molecular Weight: 642.8 Sequence: Acetyl-Arg-Leu-Arg-7-amido-4-methylcoumarin Solubility: Soluble in DMSO, methanol or water (aqueous solutions). Use: Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100uM. Activity with purified proteasome: Km=78uM. Storage and Stability: Stable for at least 1 year after receipt when stored at -20°C.