226795
Caspase-12 (CASP-12)
Size
£479.00
- SKU:
- 226795
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse caspase-12 (p20 fragment) and synthetic peptide corresponding to aa183-205 of mouse caspase-12.
- Clone:
- 15B282
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, detects a band of ~51kD Optimal dilutions to be determined by the researcher. Endotoxin: <0.1EU/ug purified protein (LAL test; Lonza). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice