226794
Caspase-11 (Caspase-11, Protease ICH-3)
Size
£544.00
- SKU:
- 226794
- Purity:
- Purified by Protein G affinity chromatography. Endotoxin: ≤0.1EU/ug (LAL test; Lonza).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to mouse caspase-11 (p20 fragment).
- Clone:
- 4E11
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml Immunoprecipitation: 1-5ug/ml ELISA: recombinant protein Flow Cytometry: overexpressed in cell lines Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice