Caspase-8 (Apoptotic Cysteine Protease, Apoptotic Protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like Protease)

Size

£479.00

SKU:: 226791
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse caspase-8 (p20 subunit).
Clone:: 15B285
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin: <0.1EU/ug purified protein (LAL test; Lonza). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice