PLC y2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, PLC-IV, Phospholipase C-gamma-2, PLC-gamma-2, PLCG2, PLCy2, PLC-y2, PLC y-2)

Size

£479.00

SKU:: 225133
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant full length Human PLC γ2.
Format:: Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Extra Details:: Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC y1, PLC y2, PLC ∂1, PLC ∂2 and PLC e. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC y2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR y chain), which bind to its C-terminal SH2 domain. PLC y1 associates with Syk in B cells, but PLC y2 does not associate with Syk in platelets. The C-terminal SH2 domain is involved in the regulation of PLC y2. In addition, Btk can induce PLC y2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence . Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200 Immunofluorescence: 1:20-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice