225113
PKR, phosphorylated (T451) (Interferon-induced, double-stranded RNA-Activated Protein Kinase, Eukaryotic Translation Initiation Factor 2-alpha Kinase 2, eIF-2A Protein Kinase 2, Interferon-inducible RNA-Dependent Protein Kinase, P1/eIF-2A Protein Kinase, Protein Kinase RNA-Activated, PKR, Tyrosine-Protein Kinase EIF2AK2, p68 Kinase, PKR, PRKR, EIF2AK2)
Size
£448.00
- SKU:
- 225113
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide derived from human PKR around the phosphorylation site of Threonine 451.
- Format:
- Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide
- Extra Details:
- An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the alpha subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2alpha on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice