219935
IL-22, Recombinant, Mouse
Size
£403.00
- SKU:
- 219935
- Molecular Weight:
- 33.4
- Purity:
- ≥98% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
- Extra Details:
- IL-22 is a member of the IL-10 family of regulatory cytokines, which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10Rb/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family. Recombinant mouse IL-22 is a 33.4kD, non-disulfide-linked, homodimeric protein consisting of two 147 amino acid polypeptide chains, expressed in E. coli. Molecular Weight: ~33.4kD Amino Acid Sequence: MLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV Biological Activity: Determined by its ability to induce STAT3 activation in COLO 205 cells. The expected ED50 for this effect is 150-400 pg/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice