219773

Follistatin, Recombinant, Human (FST, FS, Activin-binding Protein)

Size

£338.00

SKU:: 219773
Molecular Weight:: 31.5
Purity:: ≥95% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 10mM sodium phosphate, pH 7.5, 75mM sodium chloride. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Extra Details:: Follistatin is a secreted protein that binds to ligands of the TGF-beta family and regulates their activity by inhibiting their access to signaling receptors. It was originally discovered as an activin antagonist whose activity suppresses expression and secretion of the pituitary hormone FSH (follicle stimulating hormone). In addition to being a natural antagonist, follistatin can inhibit the activity of other TGF-beta ligands including BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-beta1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. Recombinant human Follistatin is a 31.5kD protein containing 288 amino acids. Its primary structure contains three cysteine-rich domains (called FS domains), each followed by a protease-inhibitory kazal domain. Molecular Weight: ~31.5kD Biological Activity: Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.1-0.4ug/ml in the presence of 7.5ng/ml Activin A. Amino Acid Sequence: GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice