218743
IgG, H&L (Agarose)
Size
£505.00
- SKU:
- 218743
- Application:
- IP
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Chicken/Avian
- Immunogen:
- Highly purified rabbit IgG
- Format:
- Supplied as a gel in PBS, pH 7.2, 0.05% sodium azide. Labeled with N-hydroxysuccinimide (NHS)-activated agarose beads.
- Extra Details:
- Chickens were immunized with highly purified rabbit IgG isolated from normal pooled rabbit serum. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbent and conjugated to conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites. Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Specific Activity: 1.1mg rabbit IgG bound/ml agarose. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: We do not recommended adding glycerol as a cryopreservative.
- Shipping Conditions:
- Blue Ice