Clostridium difficile Binary Toxin, B Subunit

Size

£665.00

SKU:: 218706
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Highly purified Binary Toxin, B subunit, purified from Clostridium difficile.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.075% sodium azide.
Extra Details:: Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics. Toxin B (TcdB) is a large exotoxin. Its role in disease is less well understood than that of toxin A, though it seems clear that TcdB contributes to disease. Both toxins modifies the Ras superfamily of small GTPases via glycosylation, inactivating them and leading to the disruption of vital signaling pathways in the cell. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: the midpoint of the dose response curve is 110ng/ml using a coating of 1ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.