4-1BB Ligand (TNFSF9, Tumor Necrosis Factor Ligand Superfamily Member 9)

Size

£704.00

SKU:: 218697
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse 4-1BB Ligand.
Clone:: 14L1
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Extra Details:: TNFSF9, a type II transmembrane glycoprotein and member of the TNF superfamily, preferentially expressed by B cells, which acts as a ligand for CD137 (4-1BB ligand receptor/TNFRSF9). Interaction between 4-1BBL and its receptor acts as a co-stimulatory signal for T cell activation and expansion, and is involved in the generation of cytotoxic T cells (Tc) and in the process of antigen presentation. Expression of 4-1BBL by a variety of carcinoma cells, suggests a role for 4-1BBL in tumor cell-T cell interactions. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:200 Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.