VAV1, Recombinant, Human, aa189-565, His-Tag (Proto-Oncogene Vav Isoform 1)

Size

£512.00

SKU:: 218667
Molecular Weight:: 46.8
Purity:: ~85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 2mM DTT, 30% glycerol.
Extra Details:: Proto-oncogene vav isoform 1, also known as VAV1, is a member of the VAV gene family. The VAV1 proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. In addition, VAV1 contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of VAV1 is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. Source: Recombinant protein corresponding to aa189-565 from human VAV1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~46.8kD (400aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.