Ung, Recombinant, E.coli, aa1-229, His-Tag (Uracil-DNA-Glycosylase)

Size

£576.00

SKU:: 218662
Molecular Weight:: 28.1
Purity:: ~90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol.
Extra Details:: Ung, also known as uracil-DNA glycosylase, is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of duMP residues. After a mutation occurs, the mutagenic threat of uracil propagates through any subsequent DNA replication steps. Source: Recombinant protein corresponding to aa1-229 from E.coli Ung, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~28.1kD (252aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.