UCK1, Recombinant, Human, aa143-273, His-Tag (Uridine-Cytidine Kinase 1 Isoform A, FLJ12255, RP11-334J6.5, URK1)

Size

£512.00

SKU:: 218658
Molecular Weight:: 17.5
Purity:: ≥80% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.4M urea, 10% glycerol.
Extra Details:: uCK1 is a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (uMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. Source: Recombinant protein corresponding to aa143-273 from human uCK1, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~17.5kD (152aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.