UBE2R2, Recombinant, Human, aa1-238, His-Tag (Ubiquitin-Conjugating Enzyme E2 R2)

Size

£576.00

SKU:: 218656
Molecular Weight:: 29.6
Purity:: ~85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 20% glycerol.
Extra Details:: UBE2R2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This protein is a protein similar to the E2 ubiquitin conjugating enzyme uBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. Source: Recombinant protein corresponding to aa1-238 from human uBE2R2, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~29.6kD (261aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.