SMCP, Recombinant, Human, aa1-116, His-Tag (Sperm Mitochondria-Associated Cysteine-Rich Protein)

Size

£512.00

SKU:: 218573
Purity:: ~85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM EDTA, 0.1mM PMSF, 20% glycerol.
Extra Details:: Sperm mitochondria differ in morphology and subcellular localization from those of somatic cells. They are elongated, flattened, and arranged circumferentially to form a helical coiled sheath in the midpiece of the sperm flagellum. SMCP localizes to the capsule associated with the mitochondrial outer membranes and is thought to function in the organization and stabilization of the helical structure of the sperm's mitochondrial sheath. Source: Recombinant protein corresponding to aa1-116 from human SMCP, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~15.2kD (139aa) (Molecular size on SDS-PAGE will appear higher) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.