SCO1, Recombinant, Human, aa132-301, His-Tag (SCO Cytochrome Oxidase Deficient Homolog 1, Protein SCO1 Homolog, Mitochondrial)

Size

£512.00

SKU:: 218539
Molecular Weight:: 20.5
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 10% glycerol.
Extra Details:: Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of cytochrome c oxidase), enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog to the yeast SCO1 gene. Source: Recombinant protein corresponding to aa132-301 from human SCO1, fused to His-tag at C-terminus, expressed in E.coli. Molecular Weight: ~20.5kD (179aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.