POLR3K, Recombinant, Human, aa1-108, His-Tag (DNA-Directed RNA Polymerase III Subunit RPC10)

Size

£512.00

SKU:: 218462
Molecular Weight:: 14.7
Purity:: ≥85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 2mM DTT, 2mM EDTA, 50% glycerol.
Extra Details:: PORL3K is a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17. Recombinant protein corresponding to aa1-108 from human PORL3K, fused to His-Tag at N-terminus, expressed in E. coli. Molecular Weight: ~14.7kD (131aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.