POLR2K, Recombinant, Human, aa1-58, His-Tag (DNA-Directed RNA Polymerases I, II, and III Subunit RPABC4)

Size

£576.00

SKU:: 218461
Molecular Weight:: 9.4
Purity:: ~85% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 1mM DTT, 10% glycerol.
Extra Details:: POLR2K is one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. Source: Recombinant protein corresponding to aa1-58 from human POLR2K, fused to His-tag at N-terminus, expressed in E.coli. Molecular Weight: ~9.4kD (81aa), confirmed by MALDI-TOF Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.